What is the recognition sequence for SmaI?
SmaI is a restriction endonuclease used to cut DNA at the recognition sequence 5′-CCC/GGG-3′, generating DNA fragments with blunt termini.
What is the recognition sequence for NotI?
The type IIP restriction enzyme NotI from Nocardia otitidis-caviarum is a homodimer which recognizes the 8 basepair DNA sequence 5′- GC/GGCCGC -3′ and cleaves both strands of DNA to create 5′, 4-base cohesive overhangs (Qiang and Schildkraut, 1987).
What is SacII?
The SacII, NEB is: Time-Saver™ qualified for digestion in 5-15 minutes. 100% activity in CutSmart® Buffer (over 210 enzymes are available in the same buffer) simplifying double digests. Supplied with 1 vial of Gel Loading Dye, Purple (6X) Requires two or more sites for cleavage.
What is the product of restriction enzymes?
Restriction enzymes, first described in 1971, are bacterially derived enzymes that cleave DNA. Evolutionarily, restriction enzymes arose as a bacterial self-defense mechanism; the genomes of invading organisms would be degraded, leading to an inability to replicate.
What type of ends does SmaI produce?
SmaI produces blunt-ended fragments whereas XmaI produces a 5´ extension.
Is NotI a restriction enzyme?
Description. Thermo Scientific NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in O buffer (Isoschizomers: CciNI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.
Where do restriction endonucleases cut DNA?
A restriction enzyme is a DNA-cutting enzyme that recognizes specific sites in DNA. Many restriction enzymes make staggered cuts at or near their recognition sites, producing ends with a single-stranded overhang. If two DNA molecules have matching ends, they can be joined by the enzyme DNA ligase.
Does Haelll leave blunt or sticky ends?
The enzyme cleaves the DNA at the positions where the GGCC sequence is found. The cleavage occurs between the second and the third nucleotides (G and C). The resulting DNA fragments are known as restriction fragments. HaeIII cuts both strands of DNA in the same location, yielding restriction fragments with blunt ends.