What grows on tryptic soy agar?
Usage of Tryptic Soy Agar (TSA) Tryptic soy agar supports the growth of nonfastidious as well as moderately fastidious microorganisms. TSA is not used for the isolation of pathogens from clinical specimens but may be used for maintaining or subculturing bacterial strains (e.g., Enterobacteriaceae and Staphylococci).
What kind of media is TSA?
TSA is the medium specified as Soybean-Casein-Digest Agar Medium by the United States Pharmacopeia (USP) for the total aerobic count portion of microbial limit testing procedures. The medium is also used for maintenance of stock cultures, plate counts and isolation of organisms from a variety of specimen types.
What type of bacteria grow on blood agar?
Blood Agar is used to grow a wide range of pathogens particularly those that are more difficult to grow such as Haemophilus influenzae, Streptococcus pneumoniae and Neisseria species. It is also required to detect and differentiate haemolytic bacteria, especially Streptococcus species.
What is the difference between EMB agar and MacConkey agar?
An example of a selective medium is MacConkey agar. It contains bile salts and crystal violet, which interfere with the growth of many gram-positive bacteria and favor the growth of gram-negative bacteria. EMB contains the dyes eosin and methylene blue that inhibit the growth of gram-positve bacteria.
What can you do with trypticase soy agar?
Trypticase soy agar. Trypticase soy agar or tryptone soya agar ( TSA) and Trypticase soy broth or tryptone soya broth ( TSB) with agar are growth media for the culturing of bacteria. They are general-purpose, nonselective media providing enough nutrients to allow for a wide variety of microorganisms to grow.
How much Tryptone is in a liter of soy agar?
One liter of the agar contains: 15 g tryptone. 5 g “soytone” – enzymatic digest of soybean meal. 5 g sodium chloride. 15 g agar. Trypticase soy agar.
Can you use soy agar instead of TSA?
Trypticase soy agar. As with any media, minor changes may be made to suit specific circumstances. TSA is frequently the base medium of other agar plate types. For example, blood agar plates (BAP) are made by enriching TSA plates with defibrinated sheep blood, and chocolate agar is made through additional cooking of BAP.
Why is TSA the base medium for agar plates?
The medium may be supplemented with blood to facilitate the growth of more fastidious bacteria or antimicrobial agents to permit the selection of various microbial groups from pure microbiota. As with any media, minor changes may be made to suit specific circumstances. TSA is frequently the base medium of other agar plate types.