What are Baf3 cells?
3 Ba/F3 cells. Cell line: Ba/F3 is a pro-B murine cell line dependent on interleukin-3 (IL-3) for growth. It is a classical cell line for investigations of cytokine receptors. It does not endogenously express the PRLR, and is absolutely dependent on cytokine receptor signaling for survival/growth.
What is Baf3?
Ba/F3, a murine interleukin-3 dependent pro-B cell line, is a popular system for exploring both kinases and their kinase inhibitors, because some protein kinases can render Ba/F3 cells to be independent of IL-3, while their inhibitors antagonize this effect. Strain.
Why use Ba F3 cells?
Ba/F3 cells are used to establish a functional interaction between Jak2 V617F and homodimeric type I cytokine receptors; it is shown that in addition to EpoR, the receptors for both thrombopoetin and G-CSF can synergize with Jak2 V617F to induce growth factor independence in Ba/F3 cells.
What is MCF 10A?
The MCF 10A cell line is a non-tumorigenic epithelial cell line. The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen. They exhibit three dimensional growth in collagen, and form domes in confluent cultures.
Why HeLa cells are so important?
HeLa cells have been used to test the effects of radiation, cosmetics, toxins, and other chemicals on human cells. They have been instrumental in gene mapping and studying human diseases, especially cancer. HeLa cells were used to maintain a culture of polio virus in human cells.
What are the advantages of CHO cell culture?
The Advantages of CHO Cells Grow well in suspension and as adherent culture, rendering the cells ideal for GMP procedures. Their tolerance to variations in pH, oxygen levels, temperature or pressure make them the ideal cell for large-scale culture. High recombinant protein yields and specific productivity.
What made Henrietta Lacks cells so special?
Lacks’ cells were different. They provided researchers with the first immortal human cell line ever grown in a laboratory. Researchers originally took HeLa cells from an aggressive cervical cancer tumour. These cells never stopped reproducing.
Are HeLa cells used today?
Today, work done with HeLa cells underpins much of modern medicine; they have been involved in key discoveries in many fields, including cancer, immunology and infectious disease. One of their most recent applications has been in research for vaccines against COVID-19.
Why are CHO cells important?
CHO cells became popular because they have a low chromosome number and were used to study genetics. These characteristics have made CHO cells the most important cell line for the production of therapeutic proteins including humanized antibodies.
Which is a caveat of DSMZ cell culture?
CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus, the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines.
Which is the best culture medium for BA / F3?
Resuspend cell pellet with the appropriate volume of complete medium, then transfer the cell suspension into a T25 culture flask. Incubate the culture at 37°, 5% CO 2 incubator or refer to the specific culture condition, if possible. 90% RPMI 1640 + 10% h.i. FBS + 10 ng/ml mouse IL-3 or 10% vol conditioned medium of WEHI-3B cell line
How many microorganisms are in the DSMZ collection?
The DSMZ microbial open collections contain over 28,800 cultures representing some 10,000 species and 2,000 genera in total. The DSMZ cell lines collection currently comprises more than 860 different immortalized cell cultures of primate, rodent, amphibian, fish, and insect origin isolated from numerous tissues as well as a number of hybridomas;
How are BA / F3 cells used in cell culture?
Ba/F3 cells are typically cultured in the presence of IL3, which stimulates the IL3 receptor and activates the JAK/STAT and mitogen-activated protein kinase (MAPK) pathways that drive proliferation and survival of the cells.