What is the difference between SuperScript III and IV?
Are there any significant changes in the SuperScript IV RT protocol compared to the SuperScript III RT protocol? The only change is that the incubation time for the reverse transcription reaction has been reduced from 50 minutes to 10 minutes. All the other parameters and steps are the same.
Why do we use random primers?
Description. Random Primers are oligodeoxyribonucleotides (mostly hexamers) used to prepare labeled DNA probes from templates for filter hybridization or in situ hybridization and to prime mRNAs with or without poly(A) for cDNA synthesis.
How does superscript III reverse transcriptase and Platinum Taq work?
The enzyme mix combines SuperScript III Reverse Transcriptase and Platinum Taq DNA Polymerase High Fidelity, which is a an enzyme mixture composed of recombinant Taq DNA polymerase, Pyrococcus species GB–D polymerase, and Platinum Taq antibodies, which block polymerase activity at ambient temperatures enabling hot start PCR.
What can superscript III reverse transcriptase do for cDNA?
SuperScript® III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme can synthesize cDNA at a temperature range of 45–60°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases.
What can superscript III RT PCR be used for?
Because SuperScript® III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA. Platinum® Taq DNA polymerase is recombinant Taq DNA polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures.
What is the function of superscript III RNA polymerase?
Reverse Transcription Enzyme Function RNA dependent DNA Polymerase Optimal Reaction Temperature 50° C Quantity 200 reactions Reverse Transcriptase SuperScript™ III Ribonuclease H Activity Reduced