How do you identify E faecalis?
In the laboratory, enterococci are distinguished by their morphologic appearance on Gram stain and culture (gram-positive cocci that grow in chains) and their ability to (1) hydrolyze esculin in the presence of bile, (2) their growth in 6.5% sodium chloride, (3) their hydrolysis of pyrrolidonyl arylamidase and leucine …
What biochemical tests are used to identify enterococcus?
The most reliable tests found here comprised the following 10 genus tests (nine out of the original 12) for the differentiation of Enterococcus: growth and reaction on bile-aesculin, Gram reaction, cell arrangement, motility, catalase production, gas from glucose, PYR, growth in the presence of 6·5% sodium chloride and …
Is Enterococcus oxidase negative?
Enterococcus faecalis is: catalase negative, oxidase negative.
What are the biochemical characteristics of Enterococcus faecalis?
Biochemical Test of Enterococcus faecalis Basic Characteristics Properties (Enterococcus faecalis ) Bile Resistance Resistance to 40% (v/v) bile Capsule Negative (-ve) Catalase Negative (-ve) Citrate Negative (-ve)
What are the expected results of TSI agar test?
Expected results of TSI Agar test are: 1 Alkaline slant/no change in butt (K/NC) i.e Red/Red = glucose, lactose and sucrose non-fermenter 2 Alkaline slant/Alkaline butt (K/K) i.e Red/Red = glucose, lactose and sucrose non-fermenter 3 Alkaline slant/acidic butt (K/A); Red/Yellow = glucose fermentation only, gas (+ or -), H 2S (+ or -)
What makes up triple sugar iron agar ( TSI )?
Triple Sugar Iron Agar. Triple sugar iron agar (TSI) is a differential medium that contains lactose, sucrose, a small amount of glucose (dextrose), ferrous sulfate, and the pH indicator phenol red.
How many species of Enterococcus are there in the world?
The genus Enterococcus consists of over 40 ecologically diverse species [5,11], yet more than 90 percent of enterococcal infections are caused by two species: E. faecalisand E. faecium[8,10,12].