What is a BSL-3 facility?

Biosafety Level 3 (BSL-3)​ BSL-3 laboratories are used to study infectious agents or toxins that may be transmitted through the air and cause potentially lethal infections. Researchers perform all experiments in a biosafety cabinet. BSL-3 laboratories are designed to be easily decontaminated.

What should be located in a BSL-3 lab?

Basic Laboratory Design for Biosafety Level 3 Laboratories

• GENERAL. The laboratory must consist of an anteroom and laboratory rooms.
• Anteroom. The anteroom must consist of two doors for access to the laboratory.
• Floors.
• Walls.
• Ceiling.
• Offices and Eating Areas.
• Doors.
• Windows.

What are BSL facilities?

A biosafety level (BSL), or pathogen/protection level, is a set of biocontainment precautions required to isolate dangerous biological agents in an enclosed laboratory facility. The levels of containment range from the lowest biosafety level 1 (BSL-1) to the highest at level 4 (BSL-4).

What are BSL-3 organisms list?

NIAID BSL-3 Priority Pathogens

• Anthrax (Bacillus anthracis)
• Brucella (Brucella abortus)
• Burkholderia.
• Botulism (Clostridium botulinum)
• Tularemia (Francisella tularensis)
• Tuberculosis (Mycobacterium tuberculosis)
• Plague (Yersinia pestis)
• Q FEVER (Coxiella burnetii)

What is the difference between bsl1 and bsl2?

The main difference in the work procedures followed in a BSL-1 laboratory and a BSL-2 laboratory is that employees in a BSL-2 laboratory will use a BSC as a primary barrier for potentially hazardous aerosols.

How much does it cost to build a BSL-3 lab?

BSL-3 lab total cost without select agent program, $88.48/nsf. BSL-3 lab total cost with select agent program, $128.70/nsf. ABSL-3 lab total cost without select agent program, $128.92/nsf.

Why is it important that a negative air pressure is maintained inside the biosafety level 3?

Preventing spread of BSL III pathogens is crucial. When a spill or accident occurs the negative pressure in the biosafety level III laboratory room prevents that these pathogens leave the laboratory room.

What are the guidelines for laboratory facilities and safety equipment?

General Laboratory Safety Rules

• Know locations of laboratory safety showers, eyewashstations, and fire extinguishers.
• Know emergency exit routes.
• Avoid skin and eye contact with all chemicals.
• Minimize all chemical exposures.
• No horseplay will be tolerated.
• Assume that all chemicals of unknown toxicity are highly toxic.

What is bsl1 and bsl2?

What is the difference between BSL-3 and 4?

BSL-4 builds upon the containment requirements of BSL-3 and is the highest level of biological safety. There are a small number of BSL-4 labs in the United States and around the world. The microbes in a BSL-4 lab are dangerous and exotic, posing a high risk of aerosol-transmitted infections.

How much does it cost to set up a laboratory?

18.5 Lakh for a mid-scale pathology lab. Approx. Rs. 1.75 Crore for large-scale pathology lab.

What is a P4 facility?

P4 Laboratories: the highest level of protection laboratory, suitable for highly hazardous to humans, animals, and plants or the environment, transmission via aerosol or unknown transmission path, or unknown, highly dangerous pathogenic factors.

Why is it important that a negative air pressure is maintained inside the biosafety level 3 lab?

What precautions and protocols are used in biosafety level 3 and 4 labs to protect personnel and prevent release of pathogens?

More protective primary barriers are used in BSL 3 laboratories, including solid-front wraparound gowns, scrub suits or coveralls made of materials such as Tyvek® and respirators as necessary. Facility design should incorporate self-closing double-door access separated from general building corridors.

What is facilities and safety?

Facilities Safety provides campus community workers with an abundance of guidance, support, awareness, and resources to conduct their workplace duties in a safe environment. Facilities Safety focuses on workplace hazards and improvements to safety measures within the workplace.